Pathways Governing Polyethylenimine Polyplex Transfection in Microporous Annealed Particle Scaffolds
نویسندگان
چکیده
منابع مشابه
Transfection of insect cell lines using polyethylenimine.
Insect cell lines have been widely used in recombinant baculovirus expression systems and transient gene expression studies. Critical to these applications have been the transfection of foreign DNA. This has been frequently done using labor intensive and cytotoxic liposome-based transfection reagents. In the current study we have optimized a new kind of polyethylenimine-based DNA transfection r...
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Linear polyethylenimine (LPEI) is an efficient transfection reagent for a broad range of cell lines and primary cells and is also useful for local or systemic gene delivery in vivo. In contrast to many other nonviral transfection methods, LPEI is less dependent on mitosis and also transfects postmitotic cells. Transfections can be performed with a simple protocol, as described here. The presenc...
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The MIT Faculty has made this article openly available. Please share how this access benefits you. Your story matters. We mechanistically explored the effect of increased hydrophobicity of the polycation on the efficacy and specificity of gene delivery in mice. N-Alkylated linear PEIs with varying alkyl chain lengths and extent of substitution were synthesized and characterized by biophysical m...
متن کاملHighly Effective Gene Transfection In Vivo by Alkylated Polyethylenimine
We mechanistically explored the effect of increased hydrophobicity of the polycation on the efficacy and specificity of gene delivery in mice. N-Alkylated linear PEIs with varying alkyl chain lengths and extent of substitution were synthesized and characterized by biophysical methods. Their in vivo transfection efficiency, specificity, and biodistribution were investigated. N-Ethylation improve...
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The aim of the present study was to optimize the polyethylenimine (PEI)-mediated transfection method in order to simplify the efficient production of lentiviral vectors (LvVs), and to compare the CaPO4- and PEI-mediated transfection methods for producing LvVs. Different titration methods of LvV stocks, as well as different culture media, culture durations, cell densities and DNA quantities were...
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ژورنال
عنوان ژورنال: Bioconjugate Chemistry
سال: 2018
ISSN: 1043-1802,1520-4812
DOI: 10.1021/acs.bioconjchem.8b00696